A properly prepared figure should be a stand alone piece of data. For example, “Figure 1 – Human RBC in 0.9% Saline (430X). The RBCs are showing the smooth biconcave morphology associated with normal RBCs.” Only figures and photographs that we have taken go into the results section, do not include any illustrations obtained from other sources. (3-4 pages long) The discussion section is where you discuss what the results mean. Did the results fit with your hypothesis? How so? You should discuss if your results were those you expected. Why did the potato cylinder lengths get longer, if they did? What was happening in the cells of the potato? Did measuring weight or lengths give similar data, or did one give more expected data than the other? Did length data correspond to the turgidity observations? Did the addition of silver nitrate inhibit osmosis, if so, how did the results demonstrate this? Based on your results would you conclude that osmosis is an example of simple diffusion or facilitated diffusion? What is facilitated diffusion? You will have to do a literature search in order to discuss the following questions. The references you use can be textbooks, primary research articles or review papers, but not general web pages, not the lab handout. Go into more detail on aquaporins and what can pass through them. What else can inhibit them? What human tissues have high concentrations of aquaporins? How is proper osmotic pressure maintained in various types of organisms that live in very salty conditions, such as salt water organisms. What is the importance of turgor pressure in plants? How is osmosis important in the functioning of the human body? What other transport mechanisms are important in living cells? The first paragraph of this lab handout gives you one reference you can use (the Niemietz paper), you are expected to find more. In this exercise you are asked to calculate the internal osmotic pressure of human RBCs and potato cells. Can you find a reference in which internal osmotic pressure of any of these was determined? Use EBSCO Host at the library’s web site to search for elodea osmosis or potato osmosis, or add other terms pertinent to our experiment, such as osmosis and aquaporins, or inhibition of aquaporins, aquaporins in potato, etc.
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