Create two primers that create restrictions sites for removal and amplification of (N-Histag-T7tag-CoVprotease-C A map of the plasmid will be provided, along with the DNA sequence of the protein, which will not have a stop or start codon within it since SARS-CoV-2 creates this protein as part of a poly protein that is cleaved up later.

Using a list of restriction enzymes in the instructions Create two primers that create restrictions sites for removal and amplification […]